Store at 2-8℃. Avoid freeze. Buffer: PBS containing 0.2% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.
After incubating HEK293T cells with 10ug/ml of puromycin for 45 minutes, cells were fixed with 4% paraformaldehyde (PFA), lysed with 0.1% triton, and then incubated with antibodies at room temperature for 20 minutes. Experimental results showed that significant fluorescent signals were detected in the puromycin-treated samples compared to the negative control group (cells without puromycin treatment but with antibody incubation), indicating that the antibody specifically recognized puromycin signal.
Puromycin is an aminonucleoside antibiotic, derived from the Streptomyces alboniger bacterium, that causes premature chain termination during translation taking place in the ribosome. It has a role as a nucleoside antibiotic, an antiinfective agent, an antineoplastic agent, a protein synthesis inhibitor, an antimicrobial agent, an EC 3.4.11.14 (cytosol alanyl aminopeptidase) inhibitor and an EC 3.4.14.2 (dipeptidyl-peptidase II) inhibitor. It is a conjugate base of a puromycin(1+).Puromycin is an antibiotic that prevents bacterial protein translation. It is utilized as a selective agent in laboratory cell cultures. Puromycin is toxic to both prokaryotic and eukaryotic cells, resulting in significant cell death at appropriate doses.
