This Kit is to detect the early stage apoptotic cell. Annexin V, a 35-36 kDa Ca2+ dependent
phospholipid-binding protein has a high affinity for phospholipid phosphatidylserine (PS),
and binds to cells with exposed PS. In apoptotic cells, the membrane PS is translocated from
the inner to the outer leaflet of the plasma membrane, exposing PS to the external cellular
environment.
Product name ABflo® 488 Annexin V/PI & Cat.No RK05875, can detect the exposed PS
through flow cytometry or fluorescence microscope.
Propidium Iodide (PI), ready-to-use nucleic acid dye, release red fluorescence light after
DNA embedding. Although PI can not penetrate the entire membrane, it can penetrate
necrotic cells or cells that have lost membrane integrity in the late stages of apoptosis.
Therefore, use of both Annexin V and PI, cells that are considered viable are Annexin V
negative and PI negative; cells that are in early apoptosis are Annexin V positive and PI
negative; and cells that are in late apoptosis or already dead are are both ABflo® 488
Annexin V and PI positive.ABflo® 488 has excitation of the light in 491nm and emission of the light in 516nm. PI-DNA
complex has maximum absorption in 535nm and maximum emission of the light in 615nm.
产品应用
Apoptosis
产品组成
Component
RK05875-50T
RK05875-100T
ABflo® 488 Annexin V
250μL
500μL
Propidium Iodide
500μL
1mL
Binding Buffer(10×)
25mL
50mL
注意事项
1. Centrifuge the reagents in the standing centrifuge tube before opening the cap.
2. Both ABflo® 488 Annexin V and PI are light sensitive
3. To avoid residual PBS affecting the experimental results, try to discard the supernatant in the last step of cell washing.
4. For accurate test results, suggest that samples should be analyzed within 1 hour after staining.
5. always wear a lab coat, lab mask and disposable gloves.
保存条件和运输
Store at 2-8℃, valid within 1 year. Avoid light preservation, and do not freeze.
