表达宿主
Baculovirus-Insect Cells
纯度
> 90 % as determined by SDS-PAGE
内毒素
< 1.0 EU per μg of the protein as determined by the LAL method
制剂
Lyophilized from sterile 20mM Tris, 1M NaCl, pH 8.0Please contact us for any concerns or special requirements.Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
描述
Recombinant Human Pleiotrophin/PTN Protein is produced by Baculovirus-Insect Cells system. The target protein is expressed with sequence (Met1-Asp168) of Human Pleiotrophin/PTN(Accession #NP_002816.1) fused with no tag.
储存
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.未开盖的干粉蛋白在 -20°C至-80°C可保存12个月；
复溶之后，蛋白溶液在-20°C及以下可保存3个月，在2-8℃可保存1周。
生物活性
Measured by its binding ability in a functional ELISA.Immobilized human PTN at 10 μg/ml (100 μl/well) can bind rat SDC1-Fc, The EC50 of rat SDC1-Fc is 0.35-0.81 μg/ml.
复溶
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.收到重组蛋白产品之后请检查蛋白冻干粉末是否贴于瓶底，如果粉末浮起，开盖之前请先低温离心。将蛋白用说明书中指定的缓冲液复溶至0.1-0.5 mg/mL（请注意蛋白复溶浓度不能低于0.1 mg/mL），室温平衡5-10 min保证充分溶解，复溶过程中请不要剧烈涡旋及吹打蛋白溶液。如需长期储存，建议复溶时添加载体蛋白或者稳定剂（如0.1% BSA, 5% HSA, 10% FBS 或者 5% 海藻糖），同时将复溶后的蛋白溶液按照需求进行分装，储存于-20°C至-80°C，随取随用，避免反复冻融。
请在蛋白复溶计算器中输入蛋白总质量和所需终浓度，快速计算您需要添加溶液的体积吧！
Background: Pleiotrophin (PTN) is a secreted, strongly heparinbinding, developmentally regulated cytokine. PTN is a highlyconserved protein ， Human, mouse, rat, canine, porcine, equine and bovine PTN share 98% aa sequenceidentity or greater. PTN and midkine share 50% amino acid (aa) sequence identity, share some functions, andconstitute a family. During development, PTN is involved in development of brain, bone, and organsundergoing branching morphogenesis. PTN causes PTPRB dimerization and inactivates its phosphatase activity,which allows increased tyrosine phosphorylation of its substrates. Increased expression of PTN is correlatedwith neuronal development or stresses such as brain ischemia and Parkinson’s disease.
